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cre dependent aav5 vector encoding channelrhodopsin paav ef1a double floxed hchr2 h134r eyfp wpre hghpa  (Addgene inc)


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    Addgene inc cre dependent aav5 vector encoding channelrhodopsin paav ef1a double floxed hchr2 h134r eyfp wpre hghpa
    Cre Dependent Aav5 Vector Encoding Channelrhodopsin Paav Ef1a Double Floxed Hchr2 H134r Eyfp Wpre Hghpa, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vector+encoding+cre/pAAV-EF1a-double+floxed-hChR2(H134R)-EYFP-WPRE-HGHpA+(Plasmid+%2320298)/bio_rxiv__64898__2026__03__11__711212-44-8-15
    Average 96 stars, based on 547 article reviews
    cre dependent aav5 vector encoding channelrhodopsin paav ef1a double floxed hchr2 h134r eyfp wpre hghpa - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Inhibition:

    Article Title: Brain motor and fear circuits regulate leukocytes during acute stress
    Article Snippet: .. For chemogenetic inhibition of glutamatergic neurons in the medulla, vGlut2 Cre mice received bilateral injections of a viral vector encoding Cre-dependent expression of the DREADD receptor hM4D(Gi)-mCherry into the medulla (bregma coordinates: −6.6 mm AP, ±1.25 mm ML, −4.32 mm DV; 1.0 μl each). pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from B. Roth (Addgene viral prep, 44362-AAV2). ..

    Expressing:

    Article Title: Brain motor and fear circuits regulate leukocytes during acute stress
    Article Snippet: .. For chemogenetic inhibition of glutamatergic neurons in the medulla, vGlut2 Cre mice received bilateral injections of a viral vector encoding Cre-dependent expression of the DREADD receptor hM4D(Gi)-mCherry into the medulla (bregma coordinates: −6.6 mm AP, ±1.25 mm ML, −4.32 mm DV; 1.0 μl each). pAAV-hSyn-DIO-hM4D(Gi)-mCherry was a gift from B. Roth (Addgene viral prep, 44362-AAV2). ..

    Article Title: Brain motor and fear circuits regulate leukocytes during acute stress
    Article Snippet: .. For chemogenetic stimulation of CRH neurons in the PVH, Crh cre mice received two injections of a viral vector encoding Cre-dependent expression of the DREADD receptor hM3D(Gq)-mCherry, or a control viral vector encoding Cre-dependent expression of mCherry only, into the PVH (bregma coordinates: −0.4 mm and −0.9 mm AP, −0.4 mm ML, −5.0 mm DV; 1 μl each). pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from B. Roth (Addgene viral prep, 44361-AAV2); pAAV-hSyn-DIO-mCherry was a gift from B. Roth (Addgene viral prep, 50459-AAV2). ..

    Plasmid Preparation:

    Article Title: Brain motor and fear circuits regulate leukocytes during acute stress
    Article Snippet: .. For chemogenetic stimulation of CRH neurons in the PVH, Crh cre mice received two injections of a viral vector encoding Cre-dependent expression of the DREADD receptor hM3D(Gq)-mCherry, or a control viral vector encoding Cre-dependent expression of mCherry only, into the PVH (bregma coordinates: −0.4 mm and −0.9 mm AP, −0.4 mm ML, −5.0 mm DV; 1 μl each). pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from B. Roth (Addgene viral prep, 44361-AAV2); pAAV-hSyn-DIO-mCherry was a gift from B. Roth (Addgene viral prep, 50459-AAV2). ..

    Control:

    Article Title: Brain motor and fear circuits regulate leukocytes during acute stress
    Article Snippet: .. For chemogenetic stimulation of CRH neurons in the PVH, Crh cre mice received two injections of a viral vector encoding Cre-dependent expression of the DREADD receptor hM3D(Gq)-mCherry, or a control viral vector encoding Cre-dependent expression of mCherry only, into the PVH (bregma coordinates: −0.4 mm and −0.9 mm AP, −0.4 mm ML, −5.0 mm DV; 1 μl each). pAAV-hSyn-DIO-hM3D(Gq)-mCherry was a gift from B. Roth (Addgene viral prep, 44361-AAV2); pAAV-hSyn-DIO-mCherry was a gift from B. Roth (Addgene viral prep, 50459-AAV2). ..



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    cre dependent aav5 vector encoding channelrhodopsin paav ef1a double floxed hchr2 h134r eyfp wpre hghpa  (Addgene inc)
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    viral vectors encoding cre recombinase  (Addgene inc)
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    Addgene inc viral vectors encoding cre recombinase
    a Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Liver SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre <t>recombinase</t> shows liver-specific protein production. b , c Cre qPCR performed on liver, lung, and spleen 1-h ( b ) or 24-h ( c ) after administration of Liver SORT LNPs at various doses. d , e Linear regression analysis comparing tdTom signal vs. 1-h qPCR ( d ) or 24-h qPCR ( e ) shows linear correlation that is modestly stronger at 1-h. f Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Lung SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase. g , h Cre qPCR performed on liver, lung, and spleen 1-h ( g ) or 24-h ( h ) after administration of Lung SORT LNPs at various doses. i , j Linear regression analysis comparing tdTom signal vs. 1-h qPCR ( i ) or 24-h qPCR ( j ) shows linear correlation that is modestly stronger at 1-h. k Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Spleen SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase shows spleen-specific protein production. l , m Cre qPCR performed on liver, lung, and spleen 1-h ( l ) or 24-h ( m ) after administration of Liver SORT LNPs at various doses. n , o Linear regression analysis comparing tdTom signal vs. 1-hour qPCR ( n ) or 24-h qPCR ( o ) shows linear correlation that is somewhat stronger at 1-h. qPCR data ( b – e , g – j , l – o ) are presented as the mean ± SEM of N = 3 mice per dose, per timepoint, per formulation; three wells per measurement. For tdTom quantification: mean ± SEM, N = 3 image fields. Correlation analyses ( d , e , i , j , n , o ) show Pearson’s R -squared with 95% CI calculated after confirming homoscedasticity. Scale bars ( a , f , k ): 200 µm.
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    a Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Liver SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase shows liver-specific protein production. b , c Cre qPCR performed on liver, lung, and spleen 1-h ( b ) or 24-h ( c ) after administration of Liver SORT LNPs at various doses. d , e Linear regression analysis comparing tdTom signal vs. 1-h qPCR ( d ) or 24-h qPCR ( e ) shows linear correlation that is modestly stronger at 1-h. f Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Lung SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase. g , h Cre qPCR performed on liver, lung, and spleen 1-h ( g ) or 24-h ( h ) after administration of Lung SORT LNPs at various doses. i , j Linear regression analysis comparing tdTom signal vs. 1-h qPCR ( i ) or 24-h qPCR ( j ) shows linear correlation that is modestly stronger at 1-h. k Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Spleen SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase shows spleen-specific protein production. l , m Cre qPCR performed on liver, lung, and spleen 1-h ( l ) or 24-h ( m ) after administration of Liver SORT LNPs at various doses. n , o Linear regression analysis comparing tdTom signal vs. 1-hour qPCR ( n ) or 24-h qPCR ( o ) shows linear correlation that is somewhat stronger at 1-h. qPCR data ( b – e , g – j , l – o ) are presented as the mean ± SEM of N = 3 mice per dose, per timepoint, per formulation; three wells per measurement. For tdTom quantification: mean ± SEM, N = 3 image fields. Correlation analyses ( d , e , i , j , n , o ) show Pearson’s R -squared with 95% CI calculated after confirming homoscedasticity. Scale bars ( a , f , k ): 200 µm.

    Journal: Nature Communications

    Article Title: Multiplexed lipid nanoparticle barcoding reveals tissue-dynamic kinetic insights and enriched cellular tropism in hepatic zones

    doi: 10.1038/s41467-025-68103-7

    Figure Lengend Snippet: a Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Liver SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase shows liver-specific protein production. b , c Cre qPCR performed on liver, lung, and spleen 1-h ( b ) or 24-h ( c ) after administration of Liver SORT LNPs at various doses. d , e Linear regression analysis comparing tdTom signal vs. 1-h qPCR ( d ) or 24-h qPCR ( e ) shows linear correlation that is modestly stronger at 1-h. f Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Lung SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase. g , h Cre qPCR performed on liver, lung, and spleen 1-h ( g ) or 24-h ( h ) after administration of Lung SORT LNPs at various doses. i , j Linear regression analysis comparing tdTom signal vs. 1-h qPCR ( i ) or 24-h qPCR ( j ) shows linear correlation that is modestly stronger at 1-h. k Endogenous Rosa-tdTom fluorescent micrographs of Ai14 mouse liver, lung, and spleen 24 h post-intravenous (IV) administration of Spleen SORT LNPs carrying 0.1, 0.25, or 1.0 mg/kg Cre recombinase shows spleen-specific protein production. l , m Cre qPCR performed on liver, lung, and spleen 1-h ( l ) or 24-h ( m ) after administration of Liver SORT LNPs at various doses. n , o Linear regression analysis comparing tdTom signal vs. 1-hour qPCR ( n ) or 24-h qPCR ( o ) shows linear correlation that is somewhat stronger at 1-h. qPCR data ( b – e , g – j , l – o ) are presented as the mean ± SEM of N = 3 mice per dose, per timepoint, per formulation; three wells per measurement. For tdTom quantification: mean ± SEM, N = 3 image fields. Correlation analyses ( d , e , i , j , n , o ) show Pearson’s R -squared with 95% CI calculated after confirming homoscedasticity. Scale bars ( a , f , k ): 200 µm.

    Article Snippet: Barcodes and a PCR adapter were cloned into an in vitro transcription (IVT) vector encoding Cre recombinase with a DNA-encoded polyA tail using Q5 SDM kit according to the manufacturer's instructions (New England Biolabs, E0554S).

    Techniques: Formulation